Genetic and Physiological Studies of Bacteriophage T5 I. An Expanded Genetic Map of T5

Genetic suppressors of bacteriophage t5 amber mutants.

Nonsense mutations in structural genes appear to abort polypeptide chain synthesis at the amino acid site governed by the mutant codon. The two presently recognized types of nonsense mutant, amber and ochre, have been assigned the codons UAG and UAA, respectively. Certain bacteria harbor one or more genetic suppressors that counteract the effect of appropriate nonsense mutations in their own st...

Polarized injection of the bacteriophage T5 chromosome.

Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.

Role of the T5 gene D15 nuclease in the generation of nicked bacteriophage T5 DNA.

The processing of newly replicated concatameric T5 DNA into both single stranded DNA changed of unit length and single-stranded fragments of sizes comparable to those found in mature T5 virion DNA occurs in the absence of late T5 protein synthesis. The formation of unit-length, single-stranded DNA chains does not require the early T5 gene D15 nuclease: however, the subsequent formation of the s...

Characterization of an unusual bipolar helicase encoded by bacteriophage T5

Bacteriophage T5 has a 120 kb double-stranded linear DNA genome encoding most of the genes required for its own replication. This lytic bacteriophage has a burst size of ∼500 new phage particles per infected cell, demonstrating that it is able to turn each infected bacterium into a highly efficient DNA manufacturing machine. To begin to understand DNA replication in this prodigious bacteriophag...

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